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goat antimice nephrin antibody  (R&D Systems)


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    R&D Systems goat antimice nephrin antibody
    Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with <t>nephrin</t> (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.
    Goat Antimice Nephrin Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+antimice+nephrin+antibody/pmc10772983-221-8-13?v=R%26D+Systems
    Average 93 stars, based on 105 article reviews
    goat antimice nephrin antibody - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue"

    Article Title: Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue

    Journal: PNAS Nexus

    doi: 10.1093/pnasnexus/pgad433

    Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.
    Figure Legend Snippet: Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.

    Techniques Used: Construct

    A 3D pathological examination detects the lesions of glomerular diseases with higher sensitivity. A) An analysis flow of anti-GBM nephritis rats. B) Representative glomerular PAS staining images in the vehicle group and anti-GBM group. Bar = 125 and 20 μm. C) Representative glomerular IF images of nephrin (green), claudin-1 (red), and DAPI (blue) in the vehicle group and anti-GBM group. The arrowheads indicate glomerular pathological lesions of crescent formation. Bar = 20 μm. D) Detection ratio of pathological lesions in the vehicle group and anti-GBM group, using conventional 2D analysis. E) The left panels show 3D overall images of glomeruli in anti-GBM group immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The middle panel shows combined images of 3D glomeruli. Bar = 10 μm. The right panels show each slice of glomeruli. Bar = 20 μm. The arrowhead indicates glomerular pathological lesions of crescent formation. F) A comparison of the detection ratio of crescent formation in the anti-GBM group between 2D and 3D analyses. * P < 0.01. G) Glomerulus volume in the vehicle group and anti-GBM group. * P < 0.01. H) Representative glomerular IF images of nephrin (green) with high magnification in the vehicle group and anti-GBM group. The lower right panel indicates the overall glomerular images immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 1 μm.
    Figure Legend Snippet: A 3D pathological examination detects the lesions of glomerular diseases with higher sensitivity. A) An analysis flow of anti-GBM nephritis rats. B) Representative glomerular PAS staining images in the vehicle group and anti-GBM group. Bar = 125 and 20 μm. C) Representative glomerular IF images of nephrin (green), claudin-1 (red), and DAPI (blue) in the vehicle group and anti-GBM group. The arrowheads indicate glomerular pathological lesions of crescent formation. Bar = 20 μm. D) Detection ratio of pathological lesions in the vehicle group and anti-GBM group, using conventional 2D analysis. E) The left panels show 3D overall images of glomeruli in anti-GBM group immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The middle panel shows combined images of 3D glomeruli. Bar = 10 μm. The right panels show each slice of glomeruli. Bar = 20 μm. The arrowhead indicates glomerular pathological lesions of crescent formation. F) A comparison of the detection ratio of crescent formation in the anti-GBM group between 2D and 3D analyses. * P < 0.01. G) Glomerulus volume in the vehicle group and anti-GBM group. * P < 0.01. H) Representative glomerular IF images of nephrin (green) with high magnification in the vehicle group and anti-GBM group. The lower right panel indicates the overall glomerular images immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 1 μm.

    Techniques Used: Staining, Comparison

    Human kidney samples could be three-dimensionally analyzed through tissue-clearing techniques. A) An analysis flow of a human-removed kidney with tissue-clearing techniques. B) The left panels show 3D images of a 1-mm-sliced human kidney sample treated with CUBIC-L&R and immunostained with nephrin (green). Bar = μm. The right panel shows each Z-stack image. Bar = 150 μm. C) The left panels show 3D overall images of human glomeruli immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The right panel shows each plane of 3D-immunostained glomerulus. D) Representative high-magnification images of human glomerulus immunostained with nephrin (green). The lower right panel indicates the overall images. Bar = 1 μm.
    Figure Legend Snippet: Human kidney samples could be three-dimensionally analyzed through tissue-clearing techniques. A) An analysis flow of a human-removed kidney with tissue-clearing techniques. B) The left panels show 3D images of a 1-mm-sliced human kidney sample treated with CUBIC-L&R and immunostained with nephrin (green). Bar = μm. The right panel shows each Z-stack image. Bar = 150 μm. C) The left panels show 3D overall images of human glomeruli immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The right panel shows each plane of 3D-immunostained glomerulus. D) Representative high-magnification images of human glomerulus immunostained with nephrin (green). The lower right panel indicates the overall images. Bar = 1 μm.

    Techniques Used:

    Paraffin-embedded human kidney samples could be three-dimensionally and noninvasively analyzed through tissue-clearing techniques. A) An analysis flow of a human paraffin-embedded kidney biopsy sample with tissue-clearing techniques. B) A 3D-reconstructed image of the whole human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red) and DAPI (blue). Bar = 200 μm. C) A 3D-reconstructed glomerulus of a human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red). and DAPI (blue). Bar = 20 μm. D) Representative high-magnification images of human glomerulus on a paraffin-embedded biopsy sample immunostained with nephrin (green). Bar = 1 μm.
    Figure Legend Snippet: Paraffin-embedded human kidney samples could be three-dimensionally and noninvasively analyzed through tissue-clearing techniques. A) An analysis flow of a human paraffin-embedded kidney biopsy sample with tissue-clearing techniques. B) A 3D-reconstructed image of the whole human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red) and DAPI (blue). Bar = 200 μm. C) A 3D-reconstructed glomerulus of a human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red). and DAPI (blue). Bar = 20 μm. D) Representative high-magnification images of human glomerulus on a paraffin-embedded biopsy sample immunostained with nephrin (green). Bar = 1 μm.

    Techniques Used:



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    Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with <t>nephrin</t> (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.
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    Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with <t>nephrin</t> (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.
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    Image Search Results


    Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.

    Journal: PNAS Nexus

    Article Title: Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue

    doi: 10.1093/pnasnexus/pgad433

    Figure Lengend Snippet: Evaluation of the suitability of tissue-clearing techniques for observing glomeruli. A) An analysis flow of a rat-dissected kidney with tissue-clearing techniques. B) Visually transparent rat kidney images treated with the respective tissue-clearing techniques. C) Representative images of 3D glomeruli immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 70 and 20 μm, respectively. D) Representative high-magnification images of 3D glomerulus immunostained with nephrin (green), which were treated with each tissue-clearing technique. Bar = 1 μm. E) 3D constructed images of a nontransparent rat kidney immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively. F) 3D constructed images of a transparent rat kidney treated with CUBIC and immunostained with nephrin (green). The right panels show each Z-depth image. Bar = 100 and 150 μm, respectively.

    Article Snippet: The primary antibodies used in this study were goat antimice nephrin antibody (AF3159; R & D Systems, Minneapolis, MN, USA), sheep antihuman nephrin antibody (AF4269; R & D Systems), and rabbit anti-claudin-1 antibody (ab15098; Abcam, Cambridge, MA, USA).

    Techniques: Construct

    A 3D pathological examination detects the lesions of glomerular diseases with higher sensitivity. A) An analysis flow of anti-GBM nephritis rats. B) Representative glomerular PAS staining images in the vehicle group and anti-GBM group. Bar = 125 and 20 μm. C) Representative glomerular IF images of nephrin (green), claudin-1 (red), and DAPI (blue) in the vehicle group and anti-GBM group. The arrowheads indicate glomerular pathological lesions of crescent formation. Bar = 20 μm. D) Detection ratio of pathological lesions in the vehicle group and anti-GBM group, using conventional 2D analysis. E) The left panels show 3D overall images of glomeruli in anti-GBM group immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The middle panel shows combined images of 3D glomeruli. Bar = 10 μm. The right panels show each slice of glomeruli. Bar = 20 μm. The arrowhead indicates glomerular pathological lesions of crescent formation. F) A comparison of the detection ratio of crescent formation in the anti-GBM group between 2D and 3D analyses. * P < 0.01. G) Glomerulus volume in the vehicle group and anti-GBM group. * P < 0.01. H) Representative glomerular IF images of nephrin (green) with high magnification in the vehicle group and anti-GBM group. The lower right panel indicates the overall glomerular images immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 1 μm.

    Journal: PNAS Nexus

    Article Title: Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue

    doi: 10.1093/pnasnexus/pgad433

    Figure Lengend Snippet: A 3D pathological examination detects the lesions of glomerular diseases with higher sensitivity. A) An analysis flow of anti-GBM nephritis rats. B) Representative glomerular PAS staining images in the vehicle group and anti-GBM group. Bar = 125 and 20 μm. C) Representative glomerular IF images of nephrin (green), claudin-1 (red), and DAPI (blue) in the vehicle group and anti-GBM group. The arrowheads indicate glomerular pathological lesions of crescent formation. Bar = 20 μm. D) Detection ratio of pathological lesions in the vehicle group and anti-GBM group, using conventional 2D analysis. E) The left panels show 3D overall images of glomeruli in anti-GBM group immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The middle panel shows combined images of 3D glomeruli. Bar = 10 μm. The right panels show each slice of glomeruli. Bar = 20 μm. The arrowhead indicates glomerular pathological lesions of crescent formation. F) A comparison of the detection ratio of crescent formation in the anti-GBM group between 2D and 3D analyses. * P < 0.01. G) Glomerulus volume in the vehicle group and anti-GBM group. * P < 0.01. H) Representative glomerular IF images of nephrin (green) with high magnification in the vehicle group and anti-GBM group. The lower right panel indicates the overall glomerular images immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 1 μm.

    Article Snippet: The primary antibodies used in this study were goat antimice nephrin antibody (AF3159; R & D Systems, Minneapolis, MN, USA), sheep antihuman nephrin antibody (AF4269; R & D Systems), and rabbit anti-claudin-1 antibody (ab15098; Abcam, Cambridge, MA, USA).

    Techniques: Staining, Comparison

    Human kidney samples could be three-dimensionally analyzed through tissue-clearing techniques. A) An analysis flow of a human-removed kidney with tissue-clearing techniques. B) The left panels show 3D images of a 1-mm-sliced human kidney sample treated with CUBIC-L&R and immunostained with nephrin (green). Bar = μm. The right panel shows each Z-stack image. Bar = 150 μm. C) The left panels show 3D overall images of human glomeruli immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The right panel shows each plane of 3D-immunostained glomerulus. D) Representative high-magnification images of human glomerulus immunostained with nephrin (green). The lower right panel indicates the overall images. Bar = 1 μm.

    Journal: PNAS Nexus

    Article Title: Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue

    doi: 10.1093/pnasnexus/pgad433

    Figure Lengend Snippet: Human kidney samples could be three-dimensionally analyzed through tissue-clearing techniques. A) An analysis flow of a human-removed kidney with tissue-clearing techniques. B) The left panels show 3D images of a 1-mm-sliced human kidney sample treated with CUBIC-L&R and immunostained with nephrin (green). Bar = μm. The right panel shows each Z-stack image. Bar = 150 μm. C) The left panels show 3D overall images of human glomeruli immunostained with nephrin (green), claudin-1 (red), and DAPI (blue). Bar = 20 μm. The right panel shows each plane of 3D-immunostained glomerulus. D) Representative high-magnification images of human glomerulus immunostained with nephrin (green). The lower right panel indicates the overall images. Bar = 1 μm.

    Article Snippet: The primary antibodies used in this study were goat antimice nephrin antibody (AF3159; R & D Systems, Minneapolis, MN, USA), sheep antihuman nephrin antibody (AF4269; R & D Systems), and rabbit anti-claudin-1 antibody (ab15098; Abcam, Cambridge, MA, USA).

    Techniques:

    Paraffin-embedded human kidney samples could be three-dimensionally and noninvasively analyzed through tissue-clearing techniques. A) An analysis flow of a human paraffin-embedded kidney biopsy sample with tissue-clearing techniques. B) A 3D-reconstructed image of the whole human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red) and DAPI (blue). Bar = 200 μm. C) A 3D-reconstructed glomerulus of a human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red). and DAPI (blue). Bar = 20 μm. D) Representative high-magnification images of human glomerulus on a paraffin-embedded biopsy sample immunostained with nephrin (green). Bar = 1 μm.

    Journal: PNAS Nexus

    Article Title: Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue

    doi: 10.1093/pnasnexus/pgad433

    Figure Lengend Snippet: Paraffin-embedded human kidney samples could be three-dimensionally and noninvasively analyzed through tissue-clearing techniques. A) An analysis flow of a human paraffin-embedded kidney biopsy sample with tissue-clearing techniques. B) A 3D-reconstructed image of the whole human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red) and DAPI (blue). Bar = 200 μm. C) A 3D-reconstructed glomerulus of a human paraffin-embedded kidney biopsy sample immunostained with nephrin (green), claudin-1 (red). and DAPI (blue). Bar = 20 μm. D) Representative high-magnification images of human glomerulus on a paraffin-embedded biopsy sample immunostained with nephrin (green). Bar = 1 μm.

    Article Snippet: The primary antibodies used in this study were goat antimice nephrin antibody (AF3159; R & D Systems, Minneapolis, MN, USA), sheep antihuman nephrin antibody (AF4269; R & D Systems), and rabbit anti-claudin-1 antibody (ab15098; Abcam, Cambridge, MA, USA).

    Techniques: